ABSTRACT
OBJECTIVE: To investigate the effects of Wenyang Huoxue Recipe (WRHXR), a compound traditional Chinese herbal medicine for warming yang and promoting blood flow, on the expression of angiopoietin mRNA in rats with chronic aristolochic acid nephropathy induced by Caulis Aristolochia Manshuriensis (CAM) decoction, and to explore the protection mechanism of WYHXR against kidney damage. METHODS: Twenty-eight male SD rats were randomly divided into normal control group, CAM group and WYHXR-treated group. Rats in the normal control group (n=8) and CAM group (n=10) were intragastrically administered with normal saline 10 ml/(kg.d) or CAM decoction 10 ml/(kg.d) respectively. Rats in the WYHXR-treated group (n=10) were intragastrically administered with WYHXR 30 g/(kg.d) and CAM decoction 10 ml/(kg.d). The expressions of Ang-l and Ang-2 mRNAs were detected by real-time reverse transcription polymerase chain reaction (RT-PCR) after 20-week treatment. RESULTS: Compared with the normal control group, the expression of Ang-l mRNA was significantly decreased, and the expression of Ang-2 mRNA was significantly increased in the CAM group (P0.05). Renal pathology showed that renal damage in WYHXR-treated group was significantly reduced as compared with the CAM group. CONCLUSION: WYHXR can up-regulate the expression of Ang-l mRNA, which may be its action mechanism in protecting the kidneys.
ABSTRACT
Objective:In occurrence and development process oflung cancer, the only known angiogenesis is one element ofthe growth, proliferation and aversion oftumor cells.VEGF, MMP-2 and MMP-9 play important role in the development and aversion ofA549 cell.This experiment is to reveal the effect ofLingqi Capsule on expression VEGF, MMP-2 and MMP-9 in A549 cell and mechanism ofanti-tumor.Methods:Medicated serum was prepared with serum-pharmacology methods, the the effect ofmedicated serum on VEGFmRNA and protein expressions ofMMP-2 and MMP-9 was detected by RT-PCR technology and immunohistochemistry respectively.Results:It suggested that the VEGF mRNAcorrect value ofthe administration group was respectively(0.804?0.020),(0.836?0.022)and(0.914?0.009),the blank control group was(0.905? 0.033)and positive control group was(0.766?0.014).Difference between the administration group and the blank control group was significant(P